For more detailed information about these platforms, click here.īegin by navigating to the FlowJo University Platforms page. The platforms provide a means for performing more sophisticated cytometric data analysis including compensation, cell cycle, proliferation, population comparison, plate editor, and kinetics to name a few. Now that you’ve gotten a handle on how FlowJo works and what types of reports it can generate, try using your data for Special Platforms. The FlowJo Basic Tutorial Video is another way to follow along. This tutorial will introduce you to basic concepts and mechanics of FlowJo vX. pdf document first ( here) and follow along. Once decompressed a new folder will be created (TQC Basic Tutorial Data) that will contain 8. Check the location where the file was saved to (usually Downloads folder on Mac, C:/Downloads or C:/Users/Downloads on PC) and double click on the file to unzip it. It will guide you through the basics of FlowJo sample organization, gating, adding statistics, and batching tables and figure layouts.Ĭlick the link labeled “The FlowJo Basic Tutorial” and wait until the file has finished downloading (83.1 MB) (see image below). The Basic Tutorial data comes from a small antibody dilution experiment. If you’re a first time user of FlowJo software you should begin by downloading the Basic Tutorial from our tutorials webpage. STEP 1: Getting Started with the Demo Data Compensation, Cell Cycle, Proliferation, Kinetics, and Population Comparison platforms are just a few of the powerful tools FlowJo provides to help you expand your flow horizons! Compensation and Special Analysis Platforms – FlowJo v10 contains various special analysis platforms to help you do advanced analysis and make adjustments.Create tabular reports with statistics and formulas from the Table Editor make graphical reports for publication, lab meeting, or to simply make a quick conclusion about your results in the Layout Editor. The Table and Layout Editors – Batch analysis begins with these two enormously powerful tools.Creating selective groups will cut down on unnecessary redundancy and get you on the fast track to making conclusions about your results. Grouping – Organizing data is key to saving time.Learn how to extract information using different graph types, how to begin gating, transform axis scaling, chase shifting populations, add statistics and much more! Graph Windows – Data analysis begins with visualizing your data.The Workspace – Learn how FlowJo organizes your data, create groups, use keywords, and prepare your data for the next steps in analysis.Note: this icon and many others were created clickable to assist with your navigation while operating FlowJo… What should I learn about first?įlowJo is a highly intuitive program for cytometric data analysis, and getting started is easy! A little time spent learning about the following components will help you save precious time and help you get more out of your data. If you’d like to test FlowJo with your own data, first get a free 30-day trial license. They’re even broken down into small sections so you can select which part of the program you’d like to learn about. Please note, the demo data files are the only files that can be used with the demo version of FlowJo. Don’t have time to read the manuals? No problem! We’ve compiled a set of short (1-5 min) video tutorials that use the same demo data to teach you how to effectively utilize the program. The tutorial’s demo data contains a set of FCS raw data and a detailed printable instruction manual (PDF, DOC, or TXT file). I downloaded FlowJo v10 now what?! Get started with some free tutorials, including demo data and learn how to do your analysis in just a few clicks.
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